In this study, we aimed to fabricate an electrochemical sensor based on copper nanoparticles and carboxylated multiwalled carbon nanotubes (MWCNTs-COOH/Cu/GCE) for the detection of resveratrol in grape seeds. Electrochemical methods including electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were employed to investigate the electrochemical behavior and performance of the sensor in a potassium ferricyanide-potassium ferrocyanide system. Differential pulse voltammetry (DPV) was used to study the electrochemical behavior of resveratrol on the sensor surface and optimize various key parameters affecting the sensor performance. The content of resveratrol was quantified using differential pulse voltammetry (DPV). Results: The highest sensitivity for resveratrol detection was achieved under the optimized conditions: a copper deposition potential of −0.4 V, a deposition time of 250s, a buffer solution pH of 7.0, and a MWCNTs-COOH dosage of 6 μL. A good linear relationship was obtained for resveratrol in the concentration range from 1×10⁻⁸ to 1×10⁻⁷ mol/L. The proposed sensor features simple preparation, excellent electrical conductivity, and high stability, providing a new method for the determination of resveratrol content.

resveratrol, MWCNT-modified electrode, electrochemical sensor